Sometimes I forget that I no longer work with humans, mice or drosophila. Working in non-model species, everything is more complicated. I'm lucky that Aziz & team have already accumulated a massive amount of knowledge on Sitophilus spp., including the tools to study them! However, one of the things that were missing was real-time RT PCR reference genes to compare gene expression across development. For this task, the entire SymSIm team came together to find the best reference genes based on this huge dataset we had previously accumulated, and that you can find here. Hopefully this will also be useful for other researchers! You can find our report here.
- ritarebollo